Tutorial

 

 

Protein band intensity / location analysis

 

This is an exemplary method that may generally work for any kind of images.

 

 

1. Original color image needs to be converted into inverted gray image

 

   
 

Original image

 

Gray image

* Apply 'Grayscaling' filter

 

Inverted image

* Apply 'Invert' filter

 

 

 

2. To calculate band intensity, analyze average gray value      * Menu > Analysis > Average gray value

 

 

For accurate analysis, it may be necessary to consider background gray value if image has gradient brightness.

 

 

3. To identify band location, analyze line profile      * Menu > Analysis > Line profile

 

 

 

4. Export tabulated data of the profile clicking on the 'Copy' button.

    Now, paste data to Excel (or other software) to analyze raw data of profile like the image shown in the below

    

 

 

 

 

 

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