Particle Analysis

Feature Particle Analysis
Feature

Particle Analysis

August 16, 2026

🦠 A Complete Guide to Particle & Colony Analysis

PhenoCapture’s Particle Color / Colony Analyzer is an advanced, automated tool designed to detect, count, and measure hundreds of individual objects in an image simultaneously. Whether you are a biologist counting bacterial colonies on an agar plate, or a material scientist analyzing particle sizes, this tool handles the heavy lifting by isolating each object and extracting highly precise size and color data.

Here is everything you need to know about automating your particle analysis.

💡 Key Features

  • Automated Detection & Counting: The software uses smart adaptive binarization to automatically find and separate individual particles or colonies, even if the lighting in your image is slightly uneven.
  • Smart Filtering: Don’t let dust or background noise ruin your data! You can set precise Min/Max Size and Min/Max Intensity limits so the software only measures the specific objects you care about.
  • Color Intensity Extraction: The tool doesn’t just count objects—it measures them. You can extract the exact color density from the Red, Green, or Blue channels, allowing you to quantify how dark or vibrant a specific colony is.
  • Visual Labeling & Overlays: The software draws a bright green outline around every valid particle and tags it with a text label directly on your image, so you know exactly which object corresponds to which data point.
  • One-Click Data Export: All the extracted data (ID, Color Intensity, Area, Mean X/Y, and Radius) is instantly organized into a neat table. Click Copy to paste everything directly into Excel for further analysis!

🛠️ How to Use the Particle & Colony Analyzer

Extracting detailed data from hundreds of particles is incredibly easy. Follow these steps:

Step 1: Set the Baseline Options

When you open the tool, select which color channel you want to analyze from the Color Channel dropdown (Red, Green, or Blue).
If your particles are darker than the background (like dark cells on a bright slide), make sure to check the Colony Darker box so the software knows how to separate them properly.

Step 2: Set Your Filters

To ensure accurate results, enter your screening conditions:

  • Min/Max Size: Filter out tiny dust particles or massive merged blobs by defining the acceptable area size.
  • Min/Max Intensity: Filter objects based on how bright or dark they are.
  • Baseline Intensity: Enter a baseline value to automatically subtract background noise from your final intensity results.

Step 3: Analyze and Preview

Click the Re-Analyze button. The software will instantly process the image.
Make sure the Preview and Labeling boxes are checked. You will immediately see bright outlines around your valid particles, complete with text labels displaying their calculated intensity.

Step 4: Export Your Data

Look at the data table at the bottom of the window to review the exact metrics for every single valid particle. When you are ready, click the Copy button to instantly transfer the tabulated data to your clipboard, ready to be pasted straight into your spreadsheet!

With the Particle & Colony Analyzer, transforming visual blobs and cells into precise, organized mathematical data is completely effortless!