PhenoCapture AI: Comprehensive Feature Summary

PhenoCapture AI provides an extensive suite of tools combining real-time image acquisition, AI-driven neural processing, advanced color adjustments, quantitative scientific measurement, and automated scripting. Below is a detailed feature breakdown organized by technical functionality.

• Modern UI Architecture: Built on the .NET with a multi-document workspace supporting simultaneous image views.
• Drag-and-Drop Interaction: Full drag-and-drop support for images, files, and multi-image preview boxes (Drag Drop Image Box & Drag Drop Image File Box), complete with Put To, Get From, and Clear commands.
• Clipboard Operations: Full Copy, Paste, and Paste and Fit to Rectangle Selection support for active selections and canvas data. Notably, when using non-rectangular selections such as Polygon, Compound Mask, or Bitmap Mask, the copied image preserves a transparent background for seamless integration into other applications.
• Advanced Selection Engine: Supports Rectangle, Circle, Polygon, and Bitmap Mask selections with Magnetic Snap & Auto-Routing for hands-free path tracing, plus custom selection box sizing (Set Selection Box Size).
• Selection Mask Operations:
  • Expand Selection and Shrink Selection by default or custom pixel amounts.
  • Reverse Selection (invert mask).
  • Create Selection From Binary Image, Add Binary Image to Selection, and Subtract Binary Image from Selection.
  • Unselect / Clear active selection masks.
• Interactive Canvas Control: Smooth zoom (In, Out, 100%, Zoom to Fit), Multiple ROIs management, UI workspace management (Reset Window Layout), and floating Toolbox panels.
• Robust Undo/Redo Engine: Dual-tracking memory engine (Undo/Redo Functions) that preserves both full image states and region selection snapshots across all operations.

• Broad Device Support: Full compatibility with WIA, WDM, and TWAIN devices (Acquire Image from Scanner, USB webcams, HD digital cameras, and 4K Ultra HD webcams up to 4096×2160 resolution).
• Hardware Compatibility & Firmware Management: Includes testing profiles for major HD webcams (Logitech Brio 4K, C910, Pro 9000, C310, C270, Microsoft LifeCam, Creative Live!). See Webcam Selection Guide.
• Broadcast-Grade Camera Controls: Deep hardware overrides for Brightness, Contrast, White Balance, Manual Exposure, Focus Lock, and PTZ via Connect to HD Camera, complete with “All Manual” lock and one-click CSV profile saving/reloading.
• Real-Time Live WebCam Viewer: Zero-lag live rendering viewer with “Always on Top” mode, and live color mapping.
• Automated Time-Lapse Capture: Time-Lapse Image Capture from local cameras, remote URLs, or screen regions with flexible scheduling (IPS, seconds, minutes, hours), automatic timestamping, and freeze-frame email alerts.
• ESP32-CAM Remote Time-Lapse Acquisition Tool: Scalable wireless multi-camera capture (based on the peer-reviewed SIPEREA platform) using sequential staggered requests to operate ~60 to ~300+ ESP32-CAM modules without Wi-Fi congestion, complete with custom time-block scheduling and auto-sorted camera subfolders.
• Remote & Network Services: Built-in Remote Webcam Server / Client, FTP Image Upload Service, and Online Live View Web Service.

• Smart Selection & Subject Matting:
  • AI Smart Selection: SAM-based bounding-box object segmentation.
  • Auto Subject Selection (Portrait): Fine-grained alpha matting tuned for human hair and clothing (MODNet).
  • Auto Subject Selection (General Object): Universal subject extraction for animals, items, and figures (U2-Net).
• AI-Powered Virtual Backgrounds: Real-time background separation without a physical green screen.
  • Multiple AI Algorithms: Choose the fast, lightweight “Selfie” model or high-resolution “Portrait” modes for incredibly sharp, accurate edge detection around hair and clothing.
  • Custom Environments: Use Solid Color Replacement to drop in a pure white background for headshots or stark green for chroma-keying. Load Custom Image Backgrounds from your local folder to transport yourself to a professional office or studio.
• AI Image Restoration & Enhancement:
  • Image Upscaling: Neural super-resolution.
  • Image Sharpening & Quality Restoration: Scalable AI models ranging from high-speed neural Image Sharpening to Image Quality Restoration with selectable performance tiers (Fastest, Fast, Slow, Slowest) for deep learning artifact and blur removal.
  • AI Inpainting: Context-aware object removal and flaw healing.
• Document & Vision AI:
  • AI Document Unwarp: Perspective correction and unwarping of curved scan pages.
  • AI OCR Text Extraction: Smart optical character recognition with structure retention.
  • AI Depth Estimation: Continuous 3D depth map generation from 2D photos.
• Cell & Binarization AI:
  • AI Binarization and Segmentation: Biological cell segmentation using robust pre-trained models.
  • Custom AI Binarization & Custom Binarization Trainer: [Built-in Training Capability] Unlike other AI tools that only perform inference with pre-trained models, this feature includes a full training engine. You can train custom UNet ONNX models using your own paired image datasets and generate your own standalone model files. Crucially, this training process is highly optimized for standard CPUs (leveraging technologies like OpenVINO CPU acceleration), delivering impressively fast training speeds without the need for expensive, high-end GPUs.

• Geometric Transforms: Flip (Horizontal/Vertical), Rotate, Crop, Resize, Resize Canvas, and Rotational Alignment.
• Spatial Filters & Noise Reduction: Stack Blur, Box Averaging, Gaussian Filter, Smart Denoise, Sharpen 3×3, Sharpen 5×5 High Quality, Advanced Sharpening, Emboss, and Mosaic Filter.
• Illumination & Background Correction: Rolling Ball Background Subtraction and Correct Uneven Illumination.
• Comprehensive Color Adjustments & Creative Color Mapping:
  • Automated Functions: Auto Tone, Auto Contrast, Auto Equalize, Auto Gamma, and Auto White Balance.
  • Color Space Controls: Hue/Saturation/Intensity (HSI), Brightness/Contrast, Gamma Adjustment, CIE L*a*b*, Multi-Band Color, Shadows/Highlights, and Adaptive Contrast.
  • Creative Color Mapping & Conversion: Apply dynamic visual styles (like thermal presets) directly to live video feeds or still images using Gray to Color (Color Map). Includes 1-click Reverse Mapping and the ability to Export the Scale (copying a beautiful high-resolution gradient straight to your clipboard). Also includes Gray Scaling, Invert (Negative colors), Color Range Selection, Change Color, Chroma Key, and Dithering to Black and White Image.
  • Channel Operations: Split RGB Channels, Gray From RGB, Delete Channel, Merge Channels, Color Deconvolution, and Convert to rg Chromaticity.

• Region & Surface Editing: Fill Region, Text Annotation, Brush Annotation (freehand drawing and highlights), Image Stamp (logo/watermark overlays), Overlay Image (alpha-blended merging), Draw Selection Border Line, Change Non-black Pixels to White, Average Image, and Differential Image.
• Binarization & Thresholding: Manual Binarization, Adaptive Binarization, Multi-Step Thresholding, and Super-Pixel Segmentation.
• Morphological Operations:
  • Erode and Dilate (3×3 to 9×9 matrix sizes).
  • Outline and Skeletonize.
  • Erode Outline, Dilate Outline, and Hide Outlines.
• Edge Detection Algorithms: Canny Edge Detection, Edge Detection (Sobel), and Edge Detection – Homogeneity.
• Binary Analysis & Clean-up: Watershed Segmentation, Hough Transform for Circles, Hough Transform for Lines, Region Coloring, Boundary Detection in Region, Binary Operations, and Remove Dots.
• Crop and Export Regions: Identify distinct regions using a binary mask and batch-export them as individual image files.
• Frequency Domain: Fast Fourier Transform (FFT) Magnitude and Inverse Fourier Transform (IFFT).

• Intensity & Spectral Analysis: Line Profile Analysis, Histogram Analysis, and Average Color Value.
• Morphometric, Counting, and Color Analysis Tools: Region Extraction Analysis, Object Color Analysis, Color Quantification, and Pixel Counter.
• Multi-Well & Array Analysis: Multiple Cell Analysis optimized for multi-well plates and micro-array grids.
• Spatial & Geometric Measurement:
  • Length Measurement (calibrated spatial distance).
  • Point Location Recorder & Plot Data Point Extractor.
  • Custom Coordinate System definition.
  • Edge Location Measurement.

• Video Frame Extraction: Extract Frames from Video File with frame-by-frame navigation, batch extraction, and clipboard copying.
• HD Video Recording: Record HD Video to MP4, WMV, or GIF with background encoding and synchronized audio.
• Video Encoding:
  • Quick Animation: Converts 2 to 5 still frames into looping animated GIFs or APNG with smart regional cropping and auto-importing.
  • Create Video From Images (Video Encoder): Converts image sequences into MP4, WMV, Animated GIF, or APNG files with custom frame rates and text overlays.
• Image Augmentation: Image Augmenter for deep learning dataset expansion.

• Built-In Code Automation: Script Runner supporting Python, JavaScript, and VBScript for custom workflow automation (API Reference).
• High-Volume Batch Processing:
  • Batch Processing: Construct multi-step action sequences from all processing modules.
  • Process single files, drag-and-drop lists, or entire folders.
  • Automatic analytical data logging to CSV or Text files.
• Batch Text Utilities: Batch Text Writer for bulk text and watermark stamping.
• Web Utilities: Automatic Image Downloader for automated web scraping.
• AI Image Classification (DNN): Seamlessly integrate ML.NET Deep Neural Networks into your batch pipelines or custom scripts. Automatically categorize and sort large datasets of images using either pre-trained models or custom models you have trained directly within PhenoCapture AI.
• AI Object Detection (YOLOv3): Harness the power of computer vision to instantly identify, count, and locate multiple objects within your images. Built into the automation engine, it supports smart tiling for high-resolution images, Non-Maximum Suppression (NMS), and direct data extraction, allowing you to fully program object-based analysis workflows.

• Standard File Support: Open File and Save As across all major formats (PNG, JPG, BMP, TIFF, GIF).
• Document & Vector Import Engine: Import Image from PDF, Import Image from Photoshop File, Scalable Vector Graphics (SVG) import, and Import Image from Office File (.docx, .xlsx, .pptx).
• Specialized Export & Print:
  • Save Selection As Transparent Image.
  • Export Grayscale to 3D STL for 3D printing and CAD modeling.
  • Print Image.
• System & Product Management: File Manager, Activate Product, direct access to MS Visual C++ Runtime Redistributables, and comprehensive viewers for Third-Party Libraries and open-source ONNX models.