PhenoCapture AI: Complete Feature Guide
Welcome to the comprehensive PhenoCapture AI Feature Guide. This guide provides an organized, menu-by-menu directory of all features, tools, and neural processing capabilities available within PhenoCapture AI, structured to strictly mirror the software’s native menu hierarchy.
1. File Menu
| Feature Name | Description |
|---|---|
| Open | Load standard raster images into the workspace.📸 |
| Save As | Save the active workspace image with custom format settings.📸 |
| Save Selection As Transparent Image | Export the selected region as an ARGB PNG with a transparent background.📸 |
| Import Image from PDF | Render and import raster images directly from PDF documents.📸 |
| Import Image from Photoshop File | Extract raster image data directly from Adobe Photoshop PSD files. |
| Import Image from Office File (docx, xlsx, pptx) | Extract embedded graphics from Word, Excel, and PowerPoint documents.📸 |
| Export Grayscale to 3D STL | Convert 2D grayscale intensity maps into 3D STL surface meshes for 3D printing.📸 |
| Send the active canvas directly to physical printers or PDF drivers.📸 | |
| Exit | Close PhenoCapture AI and release all system and hardware resources. |
2. Device Menu
| Feature Name | Description |
|---|---|
| Connect to HD Camera | Open the live webcam viewer with real-time AI background filtering.📸📸 |
| Reconnect to HD Camera | Re-establish connection to USB camera hardware. |
| Record HD Video | Capture high-definition video feeds to MP4, WMV, or animated GIF formats.📸📸 |
| Acquire Image from Scanner | Interface with TWAIN and WIA flatbed or document scanners.📸 |
| Capture | Grab a single frame from the live video feed into the workspace. |
| Capture to | Transfer live snapshots directly to specified buffer boxes. |
| Video Source | Select connected video hardware inputs. |
| Video Format | Configure resolution, frame rate, and pixel encoding modes. |
| Disconnect | Safely release hardware video drivers and camera resources. |
3. Edit Menu
| Feature Name | Description |
|---|---|
| Undo | Revert to the previous image state or selection snapshot. |
| Redo | Re-apply the next image state or selection snapshot in history. |
| Copy | Copy the active selection or canvas to the system clipboard.📸 |
| Paste | Paste image data from the clipboard onto the canvas.📸 |
| Unselect | Clear all active selection shapes and masks (Delete key). |
| Expand Selection | Dilate the selection boundary by a default pixel radius.📸 |
| Expand Selection by Pixels | Dilate the selection boundary by a user-specified pixel value.📸 |
| Shrink Selection | Erode the selection boundary by a default pixel radius.📸 |
| Shrink Selection by Pixels | Erode the selection boundary by a user-specified pixel value.📸 |
| Reverse Selection | Invert the current region selection mask across the canvas.📸 |
| Create Selection From Binary Image | Convert a black-and-white mask image into an active interactive selection.📸 |
| Add Binary Image to Selection | Merge new binary shapes into the existing selection mask. |
| Subtract Binary Image from Selection | Cut out binary shapes from the current selection mask. |
| Set Selection Box Size | Define exact numerical width and height dimensions for selection boxes.📸 |
| Put to | Push the active selection or image to a Drag-drop Image Box buffer. |
| Get from | Retrieve image content from a Drag-drop Image Box buffer. |
| Clear Drag-drop Image Box | Reset and clear memory buffer slots in the Drag-drop Image Box. |
4. Image Menu
| Feature Name | Description |
|---|---|
| Flip | Mirror canvas orientation horizontally or vertically.📸 |
| Crop | Trim the canvas strictly to the active selection boundary.📸 |
| Rotate | Reorient the image by standard 90/180 degree steps or custom angles.📸 |
| Resize | Rescale image pixel dimensions.📸 |
| Resize Canvas | Expand or trim the canvas border padding without scaling image content.📸 |
| Rotational Alignment by Straight Line | Level crooked photos by drawing a reference line along a known straight axis.📸 |
| Text Annotation | Add customizable text labels, fonts, and annotations directly onto the canvas.📸 |
| Brush Annotation | Draw, highlight, and add freehand markups directly onto your images.📸 |
| Box Averaging | Apply spatial box averaging smoothing filters across custom matrix sizes.📸 |
| Gaussian Filter | Smooth high-frequency noise using Gaussian weighted convolution matrixes.📸 |
| Smart Denoise | Suppress sensor noise while preserving sharp structural object edges.📸 |
| Sharpen 3×3 | Apply 3×3 kernel spatial edge sharpening.📸 |
| Sharpen 5×5 High Quality | Apply high-precision 5×5 kernel spatial edge sharpening.📸 |
| Advanced Sharpening | Fine-tune unsharp masking and local contrast enhancement parameters.📸 |
| Correct Uneven Illumination | Equalize non-uniform lighting gradients across sample photos. |
| Rolling Ball Background Subtraction | Remove non-uniform background intensity variations for quantitative reads.📸 |
| Emboss | Apply 3D directional light relief texture effects.📸 |
| Mosaic Filter | Pixelate selected regions or full images for privacy masking.📸 |
| Dithering to Black and White Image | Convert grayscale images into 1-bit halftoned dithered representations. |
5. Color Menu
| Feature Name | Description |
|---|---|
| Auto Tone | Automatically balance overall color tonal distribution. |
| Auto Contrast | Automatically expand image dynamic range to full contrast limits. |
| Auto Equalize | Perform histogram equalization across image brightness channels.📸 |
| Auto Gamma | Automatically adjust non-linear luminance response curves.📸 |
| Auto White Balance | Correct color temperature and balance ambient light casts. |
| CIE L*a*b* | Adjust perceptual lightness and chromaticity a and b channel. |
| Multi-Band Color | Selectively manipulate specific hue bands across the spectrum. |
| Adaptive Constrast | Enhance local contrast adaptively across shadow and highlight regions.📸 |
| Shadows/Highlights | Recover dark shadow details or clipped highlight areas locally. |
| Brightness/Contrast | Linear manual brightness and contrast level adjustments.📸 |
| Gamma Adjustment | Correct the overall exposure of your images.📸📸 |
| Hue/Saturation/Intensity | Adjust Hue, Saturation, and Intensity (HSI) color components.📸 |
| Invert | Invert color channels to negative representations. |
| Gray Scaling | Convert color images into 8-bit monochromatic grayscale. |
| Gray to Color (Color Map) | Apply false-color thermal and rainbow gradients to grayscale intensity maps. |
| Split RGB to | Separate color images into distinct Red, Green, and Blue sub-images. |
| Gray From | Extract grayscale representations from individual RGB color channels. |
| Delete Channel | Remove specific Red, Green, or Blue channels from the color space. |
| Merge Channels | Combine separate grayscale channel images back into a composite RGB image. |
| Convert to rg Chromaticity | Normalize color space against light intensity changes for plant phenotyping. |
| Color Range Selection | Sample color ranges to generate active selection masks based on color similarity. |
| Change Color | Replace target color ranges with custom replacement colors across the canvas. |
6. Neural-Processing Menu
| Feature Name | Description |
|---|---|
| Smart Selection | Bounding-box interactive object segmentation. |
| Auto Subject Selection (Portrait) | High-precision human subject extraction with hair alpha matting.📸 |
| Auto Subject Selection (General Object) | Universal object isolation for non-human subjects and products.📸📸 |
| 1 Image Upscaling | Neural super-resolution upscaling to increase resolution while sharpening details. |
| 2 Image Sharpening (Fast) | Fast AI neural edge sharpening and halo suppression. |
| 3 Image Quality Restoration (Fastest) | High-speed lightweight AI neural image restoration. |
| 4 Image Quality Restoration (Fast) | Balanced AI neural quality restoration for moderate noise and blur. |
| 5 Image Quality Restoration (Slow) | Deep neural image quality restoration for enhanced fine details. |
| 6 Image Quality Restoration (Slowest) | Maximum multi-pass deep learning restoration for severe blur and artifact removal. |
| Background Removal (Selfie) | Ultra-fast portrait background removal.📸📸 |
| Background Removal (Portrait) | Studio-grade human background removal with precise alpha matting boundaries.📸📸 |
| Background Removal (General Object) | Universal AI background removal for products, animals, and general objects.📸📸 |
| Inpainting | Context-aware AI object removal and flaw healing.📸 |
| Depth Estimation | Predict 3D surface depth maps directly from single 2D photographs.📸 |
| Document Unwarp | Flatten, deskew, and unwarp curved book page scans.📸📸 |
| OCR Text Extraction | Extract editable text from document images while retaining formatting.📸📸 |
| Binarization and Segmentation | Neural biological cell binarization and segmentation. |
| Custom Binarization | Execute dynamically loaded user-trained ONNX binarization neural networks. |
| Custom Binarization Trainer | Train custom ONNX segmentation models using paired training image datasets. |
| Image Classification | Automated neural image recognition and classification. |
7. Processing Menu
| Feature Name | Description |
|---|---|
| Fill Region | Flood-fill the selected region with a solid color.📸 |
| Draw Selection Border Line | Render visible outline strokes along active selection boundaries.📸📸 |
| Image Stamp | Insert a company logo, add a watermark, or overlay another picture onto your image.📸 |
| Overlay Image | Blend two images together using customizable transparency alpha levels. |
| Chroma Key | Isolate and knock out specific solid color backgrounds (green/blue screen).📸 |
| Manual Binarization | Convert grayscale images to 1-bit black/white using manual threshold sliders.📸 |
| Adaptive Binarization | Apply local adaptive neighborhood thresholding to handle uneven lighting. |
| Multi-Step Thresholding | Segment images into discrete intensity steps or multi-level threshold bands. |
| Super-Pixel Segmentation | Group perceptually similar neighboring pixels into atomic super-pixel clusters.📸 |
| Change Non-black Pixels to White | Convert all non-black background areas into pure white. |
| Erode | Apply grayscale or binary structural kernel erosion. |
| Dilate | Apply grayscale or binary structural kernel dilation. |
| Canny Edge Detection | Extract object boundaries using multi-stage Canny gradient operators. |
| Edge Detection | Extract spatial edges using directional Sobel gradient matrices. |
| Edge Detection – Homogenity | Detect edges based on local pixel neighborhood homogeneity variance. |
| Average Image | Compute mean pixel intensity across multiple loaded images. |
| Differential Image | Calculate pixel-by-pixel differential difference maps between two images. |
| Fast Fourier Transform (FFT) | Convert spatial images into frequency domain magnitude spectra to analyze periodic patterns. |
| Inverse Fourier Transform (IFFT) | Reconstruct a spatial image from the frequency domain |
8. Binary Menu
| Feature Name | Description |
|---|---|
| Outline | Extract 1-pixel outer perimeter contours from binary shapes. |
| Skeletonize | Reduce binary structures to topological medial-axis centerline skeletons. |
| Skeletonize – Extended | Extended thinning morphological algorithm for complex interconnected networks. |
| Region Coloring | Assign distinct pseudo-colors to isolated disconnected binary particles. |
| Erode | Erode binary foreground region boundaries. |
| Dilate | Expand binary foreground region boundaries. |
| Erode Outline | Erode outer border lines of binary shapes. |
| Dilate Outline | Expand outer border lines of binary shapes. |
| Hide Outlines | Suppress and hide binary outline overlays from canvas display. |
| Watershed Segmentation | Separate touching circular biological particles using distance-transform watershed lines. |
| Hough Transform for Circles | Detect circular shapes and boundaries mathematically in binary images. |
| Hough Transform for Lines | Detect straight lines and linear structural vectors mathematically. |
| Binary Operation | Perform logical Boolean operations (AND, OR, XOR, NOT) between binary masks. |
| Boundary Detection in Region | Extract perimeter boundaries within selected regions of interest. |
| Remove Black Dots | Filter small pinhole background noise specks below area thresholds. |
| Remove White Dots | Filter small foreground noise specks below area thresholds. |
9. Analysis Menu
| Feature Name | Description |
|---|---|
| Line profile | Plot pixel intensity distribution curves along a user-drawn line cross-section. |
| Histogram | Display pixel brightness and channel color distribution charts. |
| Region Extraction | Automatically crop and save disconnected particle regions into separate image files. |
| Particle Color Analysis | Quantify area, perimeter, shape factors, and color values for all detected particles. |
| Color Quantification | Measure percentage composition of specific color ranges across selected regions. |
| Pixel Counter | Count total foreground versus background pixels in active selection areas. |
| Average Color Value | Compute mean Red, Green, and Blue color values across active regions. |
| Multiple Cell Analysis | Batch analyze multi-well assay plates or grid cell arrays simultaneously. |
| Length Measurement | Measure spatial distances using calibrated physical unit scale markers (μm, mm, cm). |
| Custom Coordinate | Establish custom reference origin points and coordinate axes. |
| Point Location Recorder | Interactively log (X, Y) coordinates by clicking features, exporting results to CSV. |
| Edge Location Measurement | Measure exact pixel distance spacing between parallel structural edges. |
10. Tool Menu
| Feature Name | Description |
|---|---|
| Script Runner | Write, debug, and execute custom automation scripts in Python, JavaScript, or VBScript. See API Reference. |
| Batch Processing | Build multi-step action workflows to automatically process hundreds of images and export CSV data. |
| Time-lapse Image Capture | Schedule automated periodic image acquisition from cameras or screen regions. |
| Automatic Image Downloader | Automatically scrape and download image collections from web sources. |
| Online Live View Web Service | Stream live camera feeds directly to local or web browsers. |
| FTP Image Upload Service | Automatically upload captured time-lapse images to remote FTP servers. |
| Remote Webcam Server | Broadcast live camera video feeds over local IP networks. |
| Remote Webcam Client | Receive and process live camera video streams over local IP networks. |
| Quick Animation | Merge 2 to 5 still frames into looping animated GIFs with smart cropping. |
| Create Video or Animated Gif File from Images | Encode large image sequences into MP4, WMV, or Animated GIF files. |
| Batch Text Writer | Stamp custom text, watermarks, or timestamps across entire folders of photos. |
| Extract Frames from Video File | Navigate video files frame-by-frame to extract high-resolution still photos or image sequences. |
| Image Augmenter | Generate synthetic augmented image datasets (rotations, flips, noise) for AI training. |
11. View Menu
| Feature Name | Description |
|---|---|
| Zoom In | Increase canvas magnification level. |
| Zoom Out | Decrease canvas magnification level. |
| Zoom 100% | Reset canvas display magnification to 1:1 native pixel ratio. |
| Zoom to Fit | Fit the active image dimensions strictly within the workspace window. |
| Multiple ROIs | Save and manage multiple distinct Regions of Interest across the image canvas. |
| File Manager | Open the integrated thumbnail file browser to navigate image folders. |
| Drag Drop Image Box | Dockable side panel for temporary image buffer slots. |
| Drag Drop Image File Box | Dockable side panel for queuing image file paths for batch jobs. |
| Toolbox | Floating toolbox window for quick one-click access to core image processing tools.📸 |
| Selection Tool (Magnetic Snap & Auto-Routing) | Toggle interactive selection tools with magnetic edge snapping and auto-routing. 📸 |
| Reset Window Layout | Restore default panel docking positions and UI window dimensions. |
| DPI Settings | Configure high-DPI display scaling overrides for crisp rendering.📸 |
12. Help Menu
| Feature Name | Description |
|---|---|
| About | View PhenoCapture AI version build, copyright, and registration details. |
| Visit our website | Open the official PhenoCapture AI website in your default web browser. |
| View Third-Party Libraries and Models | View credit list of open-source libraries, ONNX models, and dependencies. |
| Open Third-Party License Folder | Open the local directory containing full text licenses for embedded third-party components. |
| Activate Product | Enter license key information to unlock PhenoCapture AI commercial capabilities. |
| Download MS Visual C++ Runtime Redistributables | Direct download link for required Microsoft Visual C++ runtime runtime libraries. |